emcardioНеотложная кардиология и кардиоваскулярные рискиEmergency Cardiology and Cardiovascular Risks journal2616-633XБелорусский государственный медицинский университет10.51922/2616-633X.2022.6.2.1637emcardio-81Research ArticleОригинальные научные публикацииOriginal Scientific ResearchУльтрафиолетовая фотомодификация крови у пациентов с острым коронарным синдромом без подъема сегмента ST (Часть 1). Фотохимические реакцииUltraviolet blood photomodification in patients with non-ST elevation acute coronary syndrome (Part 1). Photochemical reactionsЛаскинаО. В.LaskinaO. V.

Минск

Minsk

ЗалесскаяГ. А.ZalesskayaG. A.

Минск

Minsk

МащарН. В.MashcharN. V.

Минск

Minsk

Белорусский государственный медицинский университетBelarusian State Medical UniversityИнститут физики им. Б.И. Степанова НАН БеларусиInstitute of Physics of the National Academy of Sciences of Belarus2022230620256216371643Copyright © Ласкина О.В., Залесская Г.А., Мащар Н.В., 20252025Ласкина О.В., Залесская Г.А., Мащар Н.В.Laskina O.V., Zalesskaya G.A., Mashchar N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://emcardio.bsmu.by/jour/article/view/81

Цель. Определение фотохимических реакций, происходящих в крови пациентов с острым коронарным синдромом без подъёма сегмента ST (ОКСбпST), с последующим изучением механизмов преобразования ультрафиолетовой модификации крови (УФМК) в биологические реакции.Методы. Изучено 90 образцов крови пациентов с ОКСбпST, в комплексное лечение которых включалась УФМК с забором крови из локтевой вены и воздействием излучения ртутной лампы (аппарат «Надежда»), курс лечения составил 5 процедур, проводимых ежедневно. Сопоставлялись спектры поглощения крови и эритроцитов, результаты оптической оксиметрии, спектрофотометрии, данные общего анализа крови до УФМК, непосредственно во время отдельных процедур и через 30 минут после окончания курса.Результаты. Изучено действие УФМК на пациентов с ОКСбпST. На основании результатов спектральных и клинических исследований крови обнаружены закономерности ее фотомодификации при воздействии ультрафиолетового (УФ) излучения. Оценены спектры поглощения крови, оксигенация крови у пациентов, которым наряду с традиционной медикаментозной терапией в комплексное лечение включалось проведение пяти курсов УФМК. Отмечено, что УФ излучение приводит к фотомодификации крови, изменению парциального давления газов крови, изменению уровня оксигемоглобина и степени насыщения гемоглобина кислородом. УФМК является одним из эффективных методов немедикаментозного лечения пациентов с ОКСбпST.Заключение. УФМК, оказывая влияние на кислородный обмен в организме пациентов с ОКСбпST, повышает кислородную емкость крови, уменьшает потребность кислорода тканями. Фотоиндуцированные изменения степени насыщения гемоглобина кислородом определяются индивидуальной чувствительностью пациентов к УФ излучению и могут контролироваться насыщением венозной крови кислородом, как наилучшего способа индивидуализации терапии.

Objective. To specify photochemical reactions, occurring in the blood of patients with non-ST elevation acute coronary syndrome (NSTE-ACS), followed by the study of the mechanisms of alterations of ultraviolet blood modification (UVBM) into biological reactions.Methods. We studied 90 blood samples from patients with NSTE-ACS, receiving complex treatment that included UVBM by mercury lamp radiation with blood taken from the ulnar vein (device “Nadezhda”), the course of treatment consisted of 5 procedures conducted daily. We compared absorption spectra of blood and erythrocytes, results of optical oximetry, spectrophotometry, data of general blood analysis before UVBM, during individual procedures and 20–30 minutes after the end of the course.Results. We have studied the effect of UVBM on patients with NSTE-ACS. Based on the results of spectral and clinical studies of blood, patterns of its photomodification under the influence of ultraviolet (UV) radiation were found. The spectra of blood absorption and blood oxygenation were evaluated in patients whose complex treatment included five courses of UVBM along with traditional drug therapy. It is noted that UV radiation leads to photomodification of blood, changes in the partial pressure of blood gases, changes in the level of oxyhemoglobin and the degree of hemoglobin oxygen saturation. UVBM is proven as an effective method of non-drug treatment of patients with NSTE-ACS.Conclusion. UVBM, influencing the oxygen metabolism in patients with NSTE-ACS, increases the oxygen capacity of the blood, reduces the oxygen demand by the tissues. Photoinduced changes in the degree of hemoglobin oxygen saturation are determined by the individual sensitivity of patients to UV radiation and can be controlled by venous blood oxygenation as the best way to individualize therapy.

острый коронарный синдром без подъема сегмента STультрафиолетовая модификация кровикислородный обменnon-ST elevation acute coronary syndromeultraviolet blood modificationoxygen exchangeReferencesKnott E.K., Hancock V.K. Irradiated blood transfusion in treatment of infections. Northwest Med, 1934, vol. 33, pp. 200−204.Knott E.K., Hancock V.K. Irradiated blood transfusion in treatment of infections. Northwest Med, 1934, vol. 33, pp. 200−204.Knott E.K. Development of ultraviolet blood irradiation. Am J Surgery, 1948, vol. 76(2), pp. 165−171.Knott E.K. Development of ultraviolet blood irradiation. Am J Surgery, 1948, vol. 76(2), pp. 165−171.Karandashov V.I., Petukhov E.B. Ultrafioletovoje obluchenie krovi [UV blood irradiation]. Moscow: Medicina, 1997. (in Russian).Karandashov V.I., Petukhov E.B. Ultrafioletovoje obluchenie krovi [UV blood irradiation]. Moscow: Medicina, 1997. (in Russian).Ganelina I.E., Samojlova K.A. eds. Mechanismy vlijanija obluchenija ultrafioletovymi luchami krovi na organism cheloveka [Mechanism of the influence of blood irradiated with UV rays on organisms of humans and animals] Collection of scientific papers. Leningrad: Nauka, 1986. (in Russian).Ganelina I.E., Samojlova K.A. eds. Mechanismy vlijanija obluchenija ultrafioletovymi luchami krovi na organism cheloveka [Mechanism of the influence of blood irradiated with UV rays on organisms of humans and animals] Collection of scientific papers. Leningrad: Nauka, 1986. (in Russian).Rowen R.J. Ultraviolet blood irradiation therapy (photo-oxydation). The cure that time forgot. Int J Biosoc Med Res, 1996, vol. 14(2), pp. 115−130.Rowen R.J. Ultraviolet blood irradiation therapy (photo-oxydation). The cure that time forgot. Int J Biosoc Med Res, 1996, vol. 14(2), pp. 115−130.Moor F.B., Hoyt R.E., Levine M.G. An evaluation of hemoirradiative therapy. Arch Physical Med, 1948, vol. 29, pp. 358−365.Moor F.B., Hoyt R.E., Levine M.G. An evaluation of hemoirradiative therapy. Arch Physical Med, 1948, vol. 29, pp. 358−365.Timofeev K.V., Rylkov V.V., Shurygin A.L., Kholmogorov V.E. Spectral studies of photochemical reaction in blood. Dokl AN SSSR, 1980, vol. 255(3), pp.751−755.Timofeev K.V., Rylkov V.V., Shurygin A.L., Kholmogorov V.E. Spectral studies of photochemical reaction in blood. Dokl AN SSSR, 1980, vol. 255(3), pp.751−755.Hamblin M.R. Ultravioletо. 295-309. doi: 10.1007/978-3-319-56017-5_25.Hamblin M.R. Ultravioletо. 295-309. doi: 10.1007/978-3-319-56017-5_25.Wu X., Hu X., Hamblin M.R. Ultraviolet blood irradiation: Is it time to remember “the cure that time forgot”? J Photochem Photobiol B, 2016, vol. 157, pp. 89-96. doi: 10.1016/j.jphotobiol.2016.02.007.Wu X., Hu X., Hamblin M.R. Ultraviolet blood irradiation: Is it time to remember “the cure that time forgot”? J Photochem Photobiol B, 2016, vol. 157, pp. 89-96. doi: 10.1016/j.jphotobiol.2016.02.007.Kuzmina N.M., Maksimov N.I. High-technological medical aid in acute coronary syndrome in the Udmurt Republic. Avicenna Bull, 2017, vol. 19, no. 1, pp. 37–41.Kuzmina N.M., Maksimov N.I. High-technological medical aid in acute coronary syndrome in the Udmurt Republic. Avicenna Bull, 2017, vol. 19, no. 1, pp. 37–41.Zalesckaya G.A., Laskina O.V. Regulyatornoe vliyanie nizkointensivnogo opticheskogo izlucheniya na oksigenaziyu obluchaemoy krovi i metabolicheskie prozessy [Regulatory Effect of Low-Intensity Optical Radiation on Oxygenation of Blood Irradiated In Vivo and Metabolic Processes]. Zhurnal prikladnoy spektroskopii, 2016, vol. 83, no. 1, pp. 81-88. (in Russian).Zalesckaya G.A., Laskina O.V. Regulyatornoe vliyanie nizkointensivnogo opticheskogo izlucheniya na oksigenaziyu obluchaemoy krovi i metabolicheskie prozessy [Regulatory Effect of Low-Intensity Optical Radiation on Oxygenation of Blood Irradiated In Vivo and Metabolic Processes]. Zhurnal prikladnoy spektroskopii, 2016, vol. 83, no. 1, pp. 81-88. (in Russian).Mitkovskaya N.P., Laskina O.V., Tifi P. Nestabilnaya stenokardiya ili nestabilnye koronarnye sindromy? [Unstable angina pectoris or unstable coronary syndromes?] Neotlozhnaya kardiologiya i kardiovaskulyarnye riski, 2020, vol. 4, no. 2, pp. 944–977. (in Russian).Mitkovskaya N.P., Laskina O.V., Tifi P. Nestabilnaya stenokardiya ili nestabilnye koronarnye sindromy? [Unstable angina pectoris or unstable coronary syndromes?] Neotlozhnaya kardiologiya i kardiovaskulyarnye riski, 2020, vol. 4, no. 2, pp. 944–977. (in Russian).Laskina O.V., Zalesskaya G.A., Mitkovskaya N.P. Primenenie fotomodifikacii krovi nizkointensivnym opticheskim izlucheniem v kompleksnom lechenii pacientov s nestabil’noj stenokardiej [Application of photomodification of blood using low-intensity optical radiation in the combination treatment of patients with unstable angina]. Neotlozhnaya kardiologiya i kardiovaskulyarnye riski, 2021, vol. 5, no. 1, pp. 1145–1150. (in Russian).Laskina O.V., Zalesskaya G.A., Mitkovskaya N.P. Primenenie fotomodifikacii krovi nizkointensivnym opticheskim izlucheniem v kompleksnom lechenii pacientov s nestabil’noj stenokardiej [Application of photomodification of blood using low-intensity optical radiation in the combination treatment of patients with unstable angina]. Neotlozhnaya kardiologiya i kardiovaskulyarnye riski, 2021, vol. 5, no. 1, pp. 1145–1150. (in Russian).Rostchupkin D.I., Murina M.A. The photobiological processes in biomembranes during the action of ultraviolet radiation. Biophys, 1993, vol. 38(6), pp.1053−1068.Rostchupkin D.I., Murina M.A. The photobiological processes in biomembranes during the action of ultraviolet radiation. Biophys, 1993, vol. 38(6), pp.1053−1068.Petukhov E.B., Korneev A.A., Malutin I.G., Koshkin V.M. Snizheniye aktivnosti perekisnogo okisleniya lipidov i trombotsitarnoy agregatsii u bo’nykh s khronicheskoy arterialnoy nedostatochnostyu konechnostey pri lechenii metodom infuzii UF-obluchennoy krovi [Decreased activity of lipid peroxidation and platelet aggregation in patients with chronic arterial insufficiency of limbs in the treatment method of infusion of UV-irradiated blood]. Vestn Chirurgii, 1989, no. 5, pp. 36−39. (in Russian).Petukhov E.B., Korneev A.A., Malutin I.G., Koshkin V.M. Snizheniye aktivnosti perekisnogo okisleniya lipidov i trombotsitarnoy agregatsii u bo’nykh s khronicheskoy arterialnoy nedostatochnostyu konechnostey pri lechenii metodom infuzii UF-obluchennoy krovi [Decreased activity of lipid peroxidation and platelet aggregation in patients with chronic arterial insufficiency of limbs in the treatment method of infusion of UV-irradiated blood]. Vestn Chirurgii, 1989, no. 5, pp. 36−39. (in Russian).Paranich A.V., Sheikman V.I., Godoj E.Ch. Sostoyanie perekisnogo okisleniya lipidov posle ultrafioletovogo oblucheniya krovi i zaschitnoe dejstvie tokoferola acetata [Status of lipid peroxidation after ultraviolet irradiation of blood, and the protective effect of tocopherol acetate]. Vracheb delo, 1992, no. 5, pp. 57−61. (in Russian).Paranich A.V., Sheikman V.I., Godoj E.Ch. Sostoyanie perekisnogo okisleniya lipidov posle ultrafioletovogo oblucheniya krovi i zaschitnoe dejstvie tokoferola acetata [Status of lipid peroxidation after ultraviolet irradiation of blood, and the protective effect of tocopherol acetate]. Vracheb delo, 1992, no. 5, pp. 57−61. (in Russian).Vichriev B.S., Markelov I.M., Matveenko A.V., Tupikova S.A., Osipovich V.K., Krajnik I.V. Immunomodulirujuschee i antioksidantnoe dejstvie autotransfusii UV obluchennoj krovi pri termicheskich ozhogach [Immunomodulatory and antioxidant effects of autotransfusion of UV irradiated blood during thermal burns]. Collection of scientific papers. edss Ganelina I.E., Samojlova K.A. Leningrad: Nauka, 1986, pp. 39−49. (in Russian).Vichriev B.S., Markelov I.M., Matveenko A.V., Tupikova S.A., Osipovich V.K., Krajnik I.V. Immunomodulirujuschee i antioksidantnoe dejstvie autotransfusii UV obluchennoj krovi pri termicheskich ozhogach [Immunomodulatory and antioxidant effects of autotransfusion of UV irradiated blood during thermal burns]. Collection of scientific papers. edss Ganelina I.E., Samojlova K.A. Leningrad: Nauka, 1986, pp. 39−49. (in Russian).Zalesskaya G.A., Ulaschik V.S., Mitkovskaya N.P., Kuchinskii A.V., Laskina O.V. Spectral manifestations of photochemical reactions at therapeutical doses of blood UV irradiation in vivo. J Appl Spectrosc, 2008, vol. 75, pp. 400−405.Zalesskaya G.A., Ulaschik V.S., Mitkovskaya N.P., Kuchinskii A.V., Laskina O.V. Spectral manifestations of photochemical reactions at therapeutical doses of blood UV irradiation in vivo. J Appl Spectrosc, 2008, vol. 75, pp. 400−405.Zalesskaya G.A., Ulastchik V.S., Kalosha I.I. Spektralnye kharakteristiki krovi, obluchaemoj in vivo terapevticheskimi dozami UV izluchenija [Spectral characteristics of blood irradiated in vivo by therapeutic doses of ultraviolet radiation], Optika i spektroskopiya, 2009, vol.107, no. 4, pp. 695−702. (in Russian).Zalesskaya G.A., Ulastchik V.S., Kalosha I.I. Spektralnye kharakteristiki krovi, obluchaemoj in vivo terapevticheskimi dozami UV izluchenija [Spectral characteristics of blood irradiated in vivo by therapeutic doses of ultraviolet radiation], Optika i spektroskopiya, 2009, vol.107, no. 4, pp. 695−702. (in Russian).Zalesskaya G.A., Ulastchik V.S. Molecular mechanisms of photohemotherapy action, J Appl Spectrosc, 2009, vol. 76, no. 1, pp. 51−75.Zalesskaya G.A., Ulastchik V.S. Molecular mechanisms of photohemotherapy action, J Appl Spectrosc, 2009, vol. 76, no. 1, pp. 51−75.Zalesskaya G.A., Laskina O.V. Fotofizicheskie processy, opredeljajustchie biologicheskuju i terapevticheskuju effectivnost ultrafioletovogo obluchenija krovi [Photophysical processes that determine the biological and therapeutic efficacy of extracorporeal ultraviolet irradiation of blood], Biomedical radioeletronics, 2010, no. 3, pp. 35−41. (in Russian).Zalesskaya G.A., Laskina O.V. Fotofizicheskie processy, opredeljajustchie biologicheskuju i terapevticheskuju effectivnost ultrafioletovogo obluchenija krovi [Photophysical processes that determine the biological and therapeutic efficacy of extracorporeal ultraviolet irradiation of blood], Biomedical radioeletronics, 2010, no. 3, pp. 35−41. (in Russian).Karandashov V.I., Kvitko N.N., Slinchenko O.I., Aleksandrova N.P. Elektronno-mikroskopicheskoe issledovanie formy eritrocitov posle extrakorporalnogo ultrafioletovogo i krasnogo kogerentnogo oblucheniya krovi [Electron microscopic study forms of erythrocytes after blood irradiation by extracorporeal UV and coherent red light], Bjul experimental biologii i mediciny, 1993, no. 5, pp. 530−532. (in Russian).Karandashov V.I., Kvitko N.N., Slinchenko O.I., Aleksandrova N.P. Elektronno-mikroskopicheskoe issledovanie formy eritrocitov posle extrakorporalnogo ultrafioletovogo i krasnogo kogerentnogo oblucheniya krovi [Electron microscopic study forms of erythrocytes after blood irradiation by extracorporeal UV and coherent red light], Bjul experimental biologii i mediciny, 1993, no. 5, pp. 530−532. (in Russian).Zalesskaya G.A., Akulich N.V., Marochkov A.V., Laskina O.V., Mitkovskaya N.P. Optical methods for correction of blood oxygentransport characteristics and their biomedical applications. J Appl Spectrosc, 2010, vol.77, no. 3, pp. 451−459.Zalesskaya G.A., Akulich N.V., Marochkov A.V., Laskina O.V., Mitkovskaya N.P. Optical methods for correction of blood oxygentransport characteristics and their biomedical applications. J Appl Spectrosc, 2010, vol.77, no. 3, pp. 451−459.Bajbekov I.M., Mavlyan-Chodzhaev R.Sh., Erstkis A.G., Moskvin S.V. Erytrocyty v norme, patologii i pri lasernom vzaimodejstvii [Erythrocytes in norm, pathology and under laser irradiation]. Moscow, (2008). (in Russian).Bajbekov I.M., Mavlyan-Chodzhaev R.Sh., Erstkis A.G., Moskvin S.V. Erytrocyty v norme, patologii i pri lasernom vzaimodejstvii [Erythrocytes in norm, pathology and under laser irradiation]. Moscow, (2008). (in Russian).Potashov L.V., Cheminava R.V. Ultrafioletovoe obluchenie sobstvennoj krovi [Ultraviolet irradiation of own blood]. Vestnik khirurgii, 1982, no. 6, pp. 130−133.Potashov L.V., Cheminava R.V. Ultrafioletovoe obluchenie sobstvennoj krovi [Ultraviolet irradiation of own blood]. Vestnik khirurgii, 1982, no. 6, pp. 130−133.Lowe T. History & information on UBI technology in treatment of various disease conditions. Drsubi.com (electronic resource). 2015. Available at: drsubi.com/wp-content/uploads/2015/03/ubiwhitepaper.pdf (accessed 26.03.2015).Lowe T. History & information on UBI technology in treatment of various disease conditions. Drsubi.com (electronic resource). 2015. Available at: drsubi.com/wp-content/uploads/2015/03/ubiwhitepaper.pdf (accessed 26.03.2015).Zalesskaya G.A. Izmenenie pokazatelej oksigenacii krovi pri fotogemoterapii [Alterations of oxygenation parameters at photohemotherapy]. Photobiology and photomedicine, 2013, №1-2, pp. 56−63. (in Russian).Zalesskaya G.A. Izmenenie pokazatelej oksigenacii krovi pri fotogemoterapii [Alterations of oxygenation parameters at photohemotherapy]. Photobiology and photomedicine, 2013, №1-2, pp. 56−63. (in Russian).Zalesskaya G.A., Kalosha I.I. Photomodification of blood by laser and ultraviolet radiation: a comporative study. Biohys, 2014 vol. 59, no. 4, pp. 799−804.Zalesskaya G.A., Kalosha I.I. Photomodification of blood by laser and ultraviolet radiation: a comporative study. Biohys, 2014 vol. 59, no. 4, pp. 799−804.

The authors declare that there are no conflicts of interest present.